Scientists I, Biology

Wave Life Sciences

Wave Life Sciences

Cambridge, MA, USA
Posted on Wednesday, June 5, 2024

Job Description

Wave Life Sciences USA, Inc. seeks Scientists I, Biology for Cambridge, MA offices. Duties include: Design and conduct lab experiments for optimization of ASO’s in CNS relevant cell models, including in vitro activity evaluation and IC50 assessment, and structure activity relationship analysis; Develop siRNA platform and investigate impacts of chemistry and stereochemistry in in vitro cellular uptake and activity in CNS relevent cell models; Explore optimized chemistry optimization patterns of siRNA for productive delivery to various extra-hepatic primary cell models. Position requires a MS, or foreign equivalent, in Pharmaceutical Sciences, Bioengineering, Biomedical Sciences, Biology, or related field and 4 years’ experience in nucleic acid therapeutic research and development, or alternatively, a BS degree or foreign equivalent in Pharmaceutical Sciences, Bioengineering, Biomedical Sciences, Biology, or related field and 6 years’ experience in nucleic acid therapeutic research and development.

Experience must include the following, which may be gained concurrently:

• 4 years’ experience and expertise in discovery and research of oligonucleotide therapeutics, including 4 years’ experience using in-vitro models, and 2 years using in-vivo models relevant to CNS diseases;

• 4 years’ experience in platform development and mechanistic studies to improve the efficacy and potency of oligonucleotide therapeutics, including 4 years’ experience with ASOs and 2 years’ experience with siRNAs;

• 4 years’ experience drafting and revising Standard Operating Protocols (SOPs) for biological studies and other important documents with in-vitro sample processing for transcript and protein study, and 2 years’ experience drafting and revising SOPS with in-vivo sample processing for transcript and protein study;

• 4 years’ experience in qualitative and quantitative analysis of ASO mediated studies involving High-throughput screening (in-vitro);

• 3 years’ experience evaluating supplies and equipment to ensure operability in laboratory settings including High throughput Liquid handlers like RNA extraction kits and reagents (like enzymes, buffers), thermocyclers, and plate readers;

• 2 years’ experience evaluating supplies and equipment to ensure operability in laboratory settings including High throughput Liquid handlers like-Agilent Bravo, Formulatrix Mantis, Biotek Plate washers, and Tissue disruptor system like Tissue pulverizer and its components;

• 2 years’ experience in research and development of siRNA therapeutics for both common and rare hepatic diseases including chronic liver disease, metabolic disorders, inborn error, and metabolism;

• 2 years’ experience in qualitative and quantitative analysis with siRNA mediated studies involving High-throughput screening, PK/PD, toxicity, and target engagement (in-vivo);

• 2 years’ experience in culture and maintenance of primary cells for different extra-hepatic tissues;

• 2 years’ experience in generation and optimization of iPSC derived neurons and maintenance and culture of primary cells;

• 2 years’ experience in siRNA platform development for extra-hepatic tissue distribution and productive delivery; and

• 2 years’ experience in processing and analysis of cells and tissue samples from in-vivo studies applying an understanding in-vivo pharmacology of oligonucleotides.