Senior Research Associate: Protein/Enzyme Engineering

Twist Bioscience

Twist Bioscience

South San Francisco, CA, USA
Posted on Friday, May 12, 2023

Programmable DNA, the software of Life, is finally here! Twist Bioscience is developing a disruptive Synthetic DNA technology that will change the world, enabling widespread health and sustainability. Synthetic biologists will use our products to engineer how organisms produce cures to diseases, make everyday chemicals by using the atmosphere as the carbon source, enable plants to make their own fertilizers and to create in-vivo diagnostics that alert us when we are sick, and many more applications.

Position Summary:

Twist Biosciences is looking for a Research Associate / Senior Research Associate (Protein/Enzyme Engineering) to join our advanced development team. The candidate will work as a part of a highly collaborative team in a data-driven multidisciplinary environment in the areas of DNA data storage, diagnostics, and therapeutics.

The candidate will be involved in the execution and development of protein engineering workflow, enzyme design, and optimization as well as directed evolution of various proteins/enzymes.

The successful candidate will play a critical role in our new protein/enzyme engineering group and be responsible for high-throughput cloning, expression & purification, screening of enzyme candidates, using high throughput robotics or droplet-based transcription-translation workflows, and developing assays for protein performance under various conditions.

You will have a passion for working in a fast-paced and highly multidisciplinary environment, are very self-motivated, well-organized and a highly productive and curious scientist that can design and perform the experiments quickly and efficiently and provide and present well analyzed data to the multidisciplinary team.

What You’ll Be Doing

  • Apply directed evolution methods to engineer proteins/enzymes with novel or improved binding properties, catalytic efficiencies and other characteristics
  • Characterization of the physicochemical properties of newly evolved enzymes
  • High-Throughput Protein Purification (HTP) and large scale library screening
  • HTP assay development and optimization
  • Automation and liquid handling systems
  • Actively participate in library design discussions


  • BS or MS in molecular biology, biochemistry, chemical biology or related discipline with at least 5 (BS) or 3 (MS) years of industry experience in Biochemistry and protein/enzyme engineering
  • Experience in directed protein evolution techniques

What You’ll Bring to the Team

  • Strong wet lab skills in protein expression, purification and characterization (affinity, ion exchange, size exclusion) FPLC, SDS-PAGE etc.
  • Hands-on experience using capillary electrophoresis systems (SeqStudio and LabChip) is highly preferred
  • Experience enzymatic/kinetic assay and protein-protein/protein-substrate interaction is preferred
  • Experience advanced molecular biology techniques of variant library generation
  • Experience in critical protein structure visualization and analysis
  • Ability to handle large scale library screening and data analysis
  • Experience with automated liquid handling systems (Hamilton, Felix etc.,) is a plus
  • Knowledge of analysis of next generation DNA sequencing datasets and homology modeling. Analysis and interpretation of sequence-function relationship, interpretation of contribution of mutations to desired properties of gene variants is a plus.
  • Experience with biochemical assays using nucleoside triphosphates and oligonucleotide synthesis is a plus.
  • Data analysis and visualization skills are preferable (e.g R, Matlab, Python) but not required
  • Excellent communication (oral and written), documentation (record keeping, lab notebook, reports, SOPs), organization and interpersonal skills.

About Twist Bioscience

Twist Bioscience synthesizes genes from scratch, known as “writing” DNA. Just as children learn to both read and write, the next phase of development for the genomics revolution is the ability to write DNA.

At Twist Bioscience, we work in service of people who are changing the world for the better. In fields such as health care, agriculture, industrial chemicals and data storage, our unique silicon-based DNA Synthesis Platform provides precision at a scale that is otherwise unavailable to our customers.

Twist Bioscience Corporation is an Equal Opportunity Employer. Twist Bioscience Corporation provides equal employment opportunities to all employees and applicants for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, age, disability, genetic characteristics, or any other category protected by law.

The base cash compensation for this California-based role is below. In addition to base salary, this role is eligible for bonus, equity, and a generous benefits package. Final compensation amounts are determined by multiple factors, including candidate skill, experience, expertise, and location and may vary from the amount listed above. Compensation may be different in other locations.
San Francisco Bay Area Pay Range
$83,591$99,700 USD